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UID:186922@bioscience.fi
DTSTART;TZID=Europe/Helsinki:19700101T000000
DTEND;TZID=Europe/Helsinki:19700101T000000
DTSTAMP:20260407T084913Z
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SUMMARY:CRISPR workshop 2025
DESCRIPTION:Turku Bioscience Genome Editing Core is organizing a workshop o
 n generating knock-out and knock-in modification using CRISPR/Cas9 technol
 ogy with in vitro ribonucleoprotein complex delivery. During the course\, 
 participants will learn how to create specific genetic modifications with 
 the CRISPR/Cas9 system\, aspects of designing gRNAs and homology-directed 
 repair templates\, and they will gain hands-on experience by working with 
 293T cell lines. Participants will use their own laptop to design specific
  guideRNAs targeting their favorite gene of interest.\n\n&nbsp\;\nContent:
 \nIntroduction to CRISPR/Cas9 system\n\ngRNA and homology-directed repair 
 template design\n\nin vitro ribonucleoprotein delivery\n\nExperimental eff
 iciency analysis\nProgram and timetable:\n24.3. Theoretical background\, C
 RISPR experiment design\n\n25.3. RNP assembly and nucleofection\n\n26.3. C
 onsultation\n\n27.3. CRISPR experiment design task processing\n\n28.3. Cel
 l harvest and flow cytometry\, data analysis\nPlace:\nMedisiina D\, C209 a
 nd C235\n\nUniversity of Turku\,\n\nKiinamyllynkatu 10\,\n\n20520\, Turku\
 nParticipants:\nPhD students\, post-doctoral researchers\, researchers\n\n
 Maximum number of participants: 20\nPrice:\nFor UTU members: 100 €\n\nOt
 hers: 100 € + VAT\nRegistration deadline:\n19.3.2025.\n\nhttps://konsta.
 utu.fi/Default.aspx?tabid=88&amp\;tap=21219\nFurther information:\ngene_ed
 iting[at]bioscience.fi
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