CRISPR workshop

    Genome Editing Core is organizing a workshop on generating knock-out and knock-in modification using CRISPR/Cas9 technology with in vitro ribonucleoprotein complex delivery. During the course, participants will learn how to create specific genetic modifications with the CRISPR/Cas9 system, aspects of designing gRNAs and homology-directed repair templates, and they will gain hands-on experience by working with 293T cell lines.


    Introduction to CRISPR/Cas9 system

    gRNA and homology-directed repair template design

    in vitro ribonucleoprotein delivery

    Experimental efficiency analysis

    Program and timetable:

    13.3. 9:00 – 11:00 Theoretical background, assigning working groups

    14.3. 9:00 – 12:00 RNP assembly and nucleofection

    17.3. 9:00 – 12:00 Cell harvest and flow cytometry, data analysis


    Medisiina D, C209 and C235


    Max. 20

    PhD students, post-doctoral researchers, researchers


    100 E


    Further information: