CRISPR workshop 2024

    Turku Bioscience Genome Editing Core is organizing a workshop on generating knock-out and knock-in modification using CRISPR/Cas9 technology with in vitro ribonucleoprotein complex delivery. During the course, participants will learn how to create specific genetic modifications with the CRISPR/Cas9 system, aspects of designing gRNAs and homology-directed repair templates, and they will gain hands-on experience by working with 293T cell lines. Participants will use their own laptop to design specific guideRNAs targeting their favorite gene of interest.

    Content:

    Introduction to CRISPR/Cas9 system
    gRNA and homology-directed repair template design
    in vitro ribonucleoprotein delivery
    Experimental efficiency analysis

    Program and timetable:

    18.3. Theoretical background, CRISPR experiment design
    19.3. RNP assembly and nucleofection
    22.3. Cell harvest and flow cytometry, data analysis, CRISPR experiment design task processing

    Place:

    Medisiina D, C209 and C235
    University of Turku,
    Kiinamyllynkatu 10,
    20520, Turku

    Participants:

    PhD students, post-doctoral researchers, researchers
    Maximum number of participants: 20

    Price:

    For UTU members: 100 €
    Others: 100 € + VAT (24%)

    Registration deadline:

    10.3.2024.
    Registration via Konsta.

    Further information:

    gene_editing@biocience.fi