Genome Editing Core is organizing a workshop on generating knock-out and knock-in modification using CRISPR/Cas9 technology with in vitro ribonucleoprotein complex delivery. During the course, participants will learn how to create specific genetic modifications with the CRISPR/Cas9 system, aspects of designing gRNAs and homology-directed repair templates, and they will gain hands-on experience by working with 293T cell lines.

Content:

Introduction to CRISPR/Cas9 system

gRNA and homology-directed repair template design

in vitro ribonucleoprotein delivery

Experimental efficiency analysis

Program and timetable:

13.3. 9:00 – 11:00 Theoretical background, assigning working groups

14.3. 9:00 – 12:00 RNP assembly and nucleofection

17.3. 9:00 – 12:00 Cell harvest and flow cytometry, data analysis

Place:

Medisiina D, C209 and C235

Participants:

Max. 20

PhD students, post-doctoral researchers, researchers

Price:

100 E

Registration:

https://konsta.utu.fi/Default.aspx?tabid=88&tap=14693

Further information: gene_editing@bioscience.fi