Lentivirus production workshop 2023
Lentivirus production workshop 2023
Lentiviral vectors are popular tools for delivering specific genes to target cells. Turku Bioscience Genome Editing Core is organizing a workshop to teach participants the know-how of this method. During this one-week-long workshop, participants will generate ready-to-use lentivirus-containing supernatant to deliver the GFP gene. In parallel, they will transduce 293FT cells using previously produced similar supernatant and gain experience in determining the infectious titer and lentivirus handling in general.
Content:
Introduction to the virus laboratory, theory of 3rd generation lentivirus system
Calcium phosphate transfection
Lentivirus-containing supernatant harvest
Lentiviral transduction of 293FT cells using supernatant from transfected cells
Program and timetable:
27.3. 9:00 – 11:00 Introduction, cell seeding
28.3. 9:00 – 12:00 Calcium phosphate transfection, lentiviral transduction.
29.3. 9:00 – 10:00 Media change
30.3. 9:00 – 12:00 Supernatant collection, media change
31.3. 9:00 – 13:00 Supernatant collection, cell harvest, FACS
Venue:
Biocity 5153
Turku University,
Tykistökatu 6,
20520, Turku
Participants:
Master’s and PhD students, post-doctoral researchers, researchers
Maximum number of participants: 6
Price:
free
Registration:
6 – 18.3.2023
https://konsta.utu.fi/Default.aspx?tabid=88&tap=14782
Further information:
gene_editing@bioscience.fi