Microscopy Instrumentation

    Widefield is fundamentally any technique in which the entire specimen of interest is exposed to the light source, with the resulting image being viewed either by the observer or a camera.

    Confocal offers controllable depth of field, elimination of out-of-focus information, and the ability to collect optical sections from thick specimens.

    Super resolution improves resolution beyond the diffraction limit. Each method has a unique set of principles to achieve this.

    Light sheet allows rapid 3D and live imaging of large specimens using a unique planar illumination approach while minimizing photobleaching and phototoxicity.

    Mouse embryo imaged on Zeiss AxioZoom.v16 with Apotome.
    Mouse embryo imaged on Zeiss AxioZoom.v16 with Apotome. Sample: Salli Keinänen.

    Stereo microscopy allows low magnification observation of a sample to study the surfaces of solid specimens or to carry out close work such as dissection or microsurgery.

    Three available workstations.

    Location: BioCity A, 5th floor, office side.

    Operating System: Windows 7 Professional 64-bit

    Software:
    • FIJI
    • Ilastik
    • FlowJo V10
    • Slidebook reader
    • NIS Elements
    Operating System: Windows 7 Professional 64-bit

    Software:
    • FIJI
    • Ilastik
    • FlowJo V10
    • Bitplane Imaris 8.1.2
    • Slidebook 6 reader
    • AutoQuant X3
    • BioImageXD
    • NIS Elements
    CPU: Intel(R) Xeon(R) CPU X5650 @ 2.67GHz (6 CPUs), ~2.7GHz
    • Memory: 24GB RAM
    • Available OS Memory: 24GB RAM
    GPU: AMD FirePro V5800
    • Display Memory: 4GB
    • Dedicated Memory: 1GB
    • Shared Memory: 3GB
    Operating System: Windows 7 Professional 64-bit

    Software:
    • FIJI
    • Ilastik
    • FlowJo V10
    • Bitplane Imaris 8.1.2 full version
    • Slidebook 6
    • AutoQuant X3
    • BioImageXD
    • NIS Elements
    CPU: Intel(R) Xeon(R) CPU E5-2667 v2 @ 3.30GHz (16 CPUs), ~3.3GHz
    • Memory: 131GB RAM
    • Available OS Memory: 130GB RAM
    GPU: AMD FirePro W9100
    • Display Memory: 4GB
    • Dedicated Memory: 3GB
    • Shared Memory: 1GB