Microscopy

    Widefield is fundamentally any technique in which the entire specimen of interest is exposed to the light source, with the resulting image being viewed either by the observer or a camera.

    Confocal offers controllable depth of field, elimination of out-of-focus information, and the ability to collect optical sections from thick specimens.

    Super resolution improves resolution beyond the diffraction limit. Each method has a unique set of principles to achieve this.

    Light sheet allows rapid 3D and live imaging of large specimens using a unique planar illumination approach while minimizing photobleaching and phototoxicity.

    Stereo microscopy allows low magnification observation of a sample to study the surfaces of solid specimens or to carry out close work such as dissection or microsurgery.

    Location: BioCity A, 5th floor, office side.

    Software:
    • FIJI
    • Ilastik
    • FlowJo V10
    • Slidebook reader
    • NIS Elements 5.02
    • Icy
    • Mathlab 2020a
    CPU:
    Intel(R) Xeon(R) CPU E5-1650 v4
    • 3.60GHz (12 CPUs)
    • Memory: 16384 MB RAM
    GPU:
    NVIDIA Quadro M4000
    • Display Memory: 16057 MB
    • Dedicated Memory: 8192 MB
    Reservation System: Image Analysis PC 1
    Software:
    • FIJI
    • Ilastik
    • FlowJo V10
    • Bitplane Imaris 8.1.2
    • Slidebook 6 reader
    • AutoQuant X3
    • BioImageXD
    • NIS Elements
    CPU: Intel(R) Xeon(R) CPU X5650 @ 2.67GHz (6 CPUs), ~2.7GHz
    • Memory: 24GB RAM
    GPU: AMD FirePro V5800
    • Display Memory: 4GB
    • Dedicated Memory: 1GB
    Reservation System: Image Analysis PC 2
    Software:
    • FIJI
    • Ilastik
    • FlowJo V10
    • Bitplane Imaris 8.1.2 full version
    • Slidebook 6
    • AutoQuant X3
    • BioImageXD
    • NIS Elements
    CPU: Intel(R) Xeon(R) CPU E5-2667 v2 @ 3.30GHz (16 CPUs), ~3.3GHz
    • Memory: 131GB RAM
    GPU: AMD FirePro W9100
    • Display Memory: 4GB
    • Dedicated Memory: 3GB
    Reservation System: Image Analysis PC 3
    • Huygens Essential