3i CSU-W1 spinning disk

    The 3i (Intelligent Imaging Innovations) Marianas spinning disk confocal microscope is perfect for fast gently imaging of live samples or large fields of view. Fast z-stack acquisition together with tile options facilitates collection of large 3D data sets.
    Specialized Techniques:
    • Sensitive camera for low light live cell imaging
    • Fluorescence recovery after photobleaching (FRAP)
    • Photoactivation
    Location: BioCity, 5th floor, room 5149

    10x Zeiss Plan-Apochromat
    • Numerical Aperture: 0.45
    • Working Distance: 2 mm
    • Immersion: Without immersion

    20x Zeiss Plan-Apochromat
    • Numerical Aperture: 0.8
    • Working Distance: 0.55 mm
    • Immersion: Without immersion

    40x Zeiss LD C-Apochromat
    • Numerical Aperture: 1.1
    • Working Distance: 0.62 mm at cover glass 0.17 mm
    • Immersion: Water

     

    63x Zeiss LD Plan-NEOFLUAR
    • Numerical Aperture: 0.75
    • Working Distance: 1.7 mm at cover glass 0.75 mm
    • Immersion: Without immersion

    63x Zeiss Plan-Apochromat
    • Numerical Aperture: 1.4
    • Working Distance: 0.19 mm
    • Immersion: Oil

    100x Zeiss Plan-Apochromat
    • Numerical Aperture: 1.4
    • Working Distance: 0.17 mm
    • Immersion: Oil

     
    • 405nm
    • 488nm
    • 561nm
    • 640nm
    • 730nm
    • Dapi   (445/45nm)
    • GFP (525/30nm)
    • Cy3/Alexa 568 (617/73nm or 595/30nm)
    • Cy5/Alexa647 (692/40nm)
    • Alexa 750, IR (809/81nm)
    • Piezo stage - ASI
    • Definite focus
    • Environment chamber - Okolab
    • Temperature control - Okolab