Zeiss AxioZoom.V16

    The instrument can perform three colour fluorescence imaging with a motorized turret. There is also optical sectioning possibility (3D) with the ApoTome.2. It has two light sources for brightfield imaging, a ring light from above and light below the sample.

    Reservation System: Zeiss AxioZoom.V16

    Location: BioCity, 5th floor, room 5062

    0.5x PlanApo Z
    • Numerical Aperture: 0.125
    • Working Distance: 114mm
    • Magnification range: 3.5x-56x
    • Field of view range: 66-4mm
    • Apotome compatible: No
    1.0x PlanApo Z
    • Numerical Aperture: 0.125
    • Working Distance: 56mm
    • Magnification range: 7x-112x
    • Field of view range: 33-2mm
    • Apotome: Yes from 40x-112x
    Two White light sources :
    • Above: CL 9000 LED CAN ring light
    • Below: visiLED MC1000
    Fluorescence Excitation:
    • HXP 200C mercury lamp
    DAPI - filter set 49
    Excitation: G365nm
    Emission: BP 445/50nm

    Alexa 488 - filter set 38 HE
    Excitation: BP 470/40nm
    Emission: BP 525/50nm
    Alexa 568 - filter set 45
    Excitation: BP 560/40nm
    Emission: BP 630/75nm

    Alexa 647 - filter set 50
    Excitation: BP 640/30nm
    Emission: 690/50nm

    With structured illumination (SIM), you know that only the focal plane appears in your image: ApoTome.2 recognizes the magnification and moves the appropriate grid into the beampath. The system then calculates your optical section from three (or more) images with different grid positions. This removes scattered out-of-focus light from your images, even in thicker specimens.

    • Zen Pro