Zeiss TIRF

    Total Internal Reflection (TIRF) microscopy is a technique, which allows imaging of fluorophores near of the basal cell membrane with high resolution in Z-axis. Approximately 70-150 nm -axis resolution can be achieved, while in common confocal microscopy the limit is approximately 600nm.
    TIRF is designed for cell membrane studies, such as endosytosis and integrin trafficking. However,  it is very suitable for all kinds of sensitive wide field microscopy, due to high quality objectives and sensitive cameras.

    Our TIRF has two different cameras dedicated to different applications: a very sensitive emccd camera with a 512×512 chip, and a sensitive and fast high resolution sCMOS camera. The microscope is equipped with incubation elements, motorized stage and Zeiss Definite Focus drift correction device.

    Location: BioCity, 2nd floor, room 2209

    2.5x Zeiss EC Epilan-Neofluar
    • Numerical Aperture: 0.06
    • Working Distance: 15.1 mm
    • Immersion: Without immersion

    10x Zeiss EC Epilan-Neofluar
    • Numerical Aperture: 0.3
    • Working Distance: 5.2 mm
    • Immersion: Without immersion

    20x Zeiss Plan-Apochromat
    • Numerical Aperture: 0.45
    • Working Distance: 8.2-6.9 mm
    • Immersion: Without immersion
    63x Zeiss α Plan-Apochromat
    • Numerical Aperture: 1.46
    • Working Distance: 0.55 mm
    • Immersion: Oil

    100x Zeiss α Plan-Apochromat
    • Numerical Aperture: 1.46
    • Working Distance: 0.11 mm
    • Immersion: Oil
    • 488nm
    • 561nm
    • 639nm

    GFP - filter set 38 HE
    Excitation: BP 470/40nm
    Emission: BP 525/50nm

    DsRed - filter set 43 HE
    Excitation: BP 550/25nm
    Emission: BP 605/70nm

    CFP/GFP/DsRed - filter set 76 HE
    Excitation: TBP 406 + 489 + 561nm
    Emission: TBP 459 + 525 + 608nm

    GFP/mRFP/Alexa633 - filter set 77 HE
    Excitation: TBP 483 + 564 + 642nm
    Emission: TBP 526 + 601 + 688

    • Hamamatsu ORCA Flash4 CMOS
    • 2048x2048 pixels
    • Definite Focus
    • Heated incubator
    • Heated sample holder