Description of the Assay
This assay can measure more than 40 bile acids and bile acid conjugates from biological fluids and tissues. Bile acids, a group of sterols which are end-products of cholesterol metabolism, are primarily involved in dietary lipid absorption and cholesterol homeostasis. Bile acids also act as signalling molecules, regulate systemic endocrine functions and their systemic composition reflects gut microbial metabolism. We have an established, quantitative method for profiling bile acid composition in various sample types including serum, faeces and body tissues. Please don’t hesitate to contact us with any questions.
Jäntti, S.E., Kivilompolo, M., Öhrnberg, L. et al. Anal Bioanal Chem (2014) 406: 7799. https://doi.org/10.1007/s00216-014-8230-9
Sample Types Accepted
- Whole blood
- Serum (see note below)
- Adipose tissue
- Faecal / intestine contents
- Bile / gallbladder tissue
- Cerebrospinal fluid(CSF)
- Cytoplasmic fraction of the brain
Generic Sample Handling Guidelines
- Samples should always be stored at -80°C and transported in dry ice.
- Ideally, samples should be aliquoted into suitably-sealed, standard containers with labels, to avoid multiple freeze thaws (e.g. 1.5/2mL Eppendorf tubes, or larger volumes in Falcon tubes).
- Sample lists/grids should be provided in electronic format along with the samples.
- If possible, provide information on sample collection and handling procedures, including :
- Sample source
- Sample collection date
- Freezing method, length of storage and temperature
- Number and type of freeze and thaw cycles
- Ideally EDTA plasma tubes should be mixed five times directly after sampling.
- Blood samples need to be kept at 4°C during preparation.
- Recommended centrifugation parameters for serum and plasma are 15 minutes at 1600g at 4°C.
- Serum standing time recommended as 30 minutes at 4°C.
- White adipose tissue samples should be taken from subcutaneous peri-umbilical fat.
- Tissues should be frozen immediately in liquid nitrogen after sampling.
- Intact faecal samples should be collected, kept at 4°C for no longer than 24 h. For any longer, it should be stored at −80°C and transported using dry ice. If such a freezer is not available, intact faecal samples can also be stored in a −20°C freezer for no more than 24 h.