Prediction of Outcome in TBI
Description of the Assay
This assay measures blood-based biomarker metabolites and related structures from blood samples. These biomarkers play a key role in determining the severity of Traumatic Brain Injury (TBI) cases. *1 The current assay, under development, can predict the outcome of a mild TBI. Additionally, this assay measures metabolic markers which can also predict the necessity of a CT scan following a TBI. *2
Please don’t hesitate to contact us with any questions in advance.
*1 (Orešič M, Posti JP, Kamstrup-Nielsen MH, et al. Human Serum Metabolites Associate With Severity and Patient Outcomes in Traumatic Brain Injury. EBioMedicine. 2016;12:118-126. doi:10.1016/j.ebiom.2016.07.015.)
*2 Dr. Alex Mountfort Dickens, Dr. Jussi P Posti, Dr. Riikka SK Takala, Dr. Henna Maria Ala-Seppälä, Dr. Ismo Mattila, Dr. Jonathan Coles Coles, Dr. Janek Frantzén, Prof. Peter John Hutchinson, Dr. Ari J Katila, Dr. Anna Kyllönen, Dr. Henna-Riikka Maanpää, Dr. Virginia Newcombe, Mrs. Joanne Outtrim, Mr. Jussi Tallus, Dr. Keri Carpenter, Dr. David Menon, Prof. Tuulia Hyotylainen, Dr. Olli Tenovuo, and Dr. Matej Oresic.Journal of Neurotrauma. http://doi.org/10.1089/neu.2017.5272
Sample Types Accepted
- Whole blood
- Serum (see note below)
Sample Handling Guidelines
- Ideally EDTA plasma tubes should be mixed five times directly after sampling.
- Blood samples need to be kept at 4 °C during preparation.
- Centrifuging parameters for serum and plasma 15 min in 1600g at 4 °C.
- Samples should always be stored at -80°C and transported in dry ice.
- Ideally, sample should be aliquoted into suitable sealed standard containers with labels, to avoid multiple freeze thaws (1.5/2mL Eppendorf-tubes, or larger volumes in Falcon tubes).
- Sample lists/grids should be provided in electronic format along with the samples.
- If possible, provide information on sample collection and handling procedures, including:
- Sample source
- Sample collection date
- Freezing method, length of storage and temperature
- Number and type of freeze and thaw cycles
For serum samples, room temperature clotting time must be controlled and consistent (ideally 30 minutes).