Prediction of Outcome in TBI

    Description of the Assay

    This assay measures blood-based biomarker metabolites and related structures from blood samples. These biomarkers play a key role in determining the severity of Traumatic Brain Injury (TBI) cases. *1 The current assay, under development, can predict the outcome of a mild TBI. Additionally, this assay measures metabolic markers which can also predict the necessity of a CT scan following a TBI. *2

    Please don’t hesitate to contact us with any questions in advance.

    *1 (Orešič M, Posti JP, Kamstrup-Nielsen MH, et al. Human Serum Metabolites Associate With Severity and Patient Outcomes in Traumatic Brain Injury. EBioMedicine. 2016;12:118-126. doi:10.1016/j.ebiom.2016.07.015.)

    *2 Dr. Alex Mountfort Dickens, Dr. Jussi P Posti, Dr. Riikka SK Takala, Dr. Henna Maria Ala-Seppälä, Dr. Ismo Mattila, Dr. Jonathan Coles Coles, Dr. Janek Frantzén, Prof. Peter John Hutchinson, Dr. Ari J Katila, Dr. Anna Kyllönen, Dr. Henna-Riikka Maanpää, Dr. Virginia Newcombe, Mrs. Joanne Outtrim, Mr. Jussi Tallus, Dr. Keri Carpenter, Dr. David Menon, Prof. Tuulia Hyotylainen, Dr. Olli Tenovuo, and Dr. Matej Oresic.Journal of Neurotrauma.

    Sample Information

    Sample Types Accepted

    • ­Plasma
    • ­Whole blood
    • ­Serum (see note below)


    Sample Handling Guidelines

    • Ideally EDTA plasma tubes should be mixed five times directly after sampling.
    • Blood samples need to be kept at 4 °C during preparation.
    • Centrifuging parameters for serum and plasma 15 min in 1600g at 4 °C.
    • Samples should always be stored at -80°C and transported in dry ice.
    • Ideally, sample should be aliquoted into suitable sealed standard containers with labels, to avoid multiple freeze thaws (1.5/2mL Eppendorf-tubes, or larger volumes in Falcon tubes).
    • Sample lists/grids should be provided in electronic format along with the samples.
    • If possible, provide information on sample collection and handling procedures, including:
      • Sample source
      • Sample collection date
      • Freezing method, length of storage and temperature
      • Number and type of freeze and thaw cycles

    For serum samples, room temperature clotting time must be controlled and consistent (ideally 30 minutes).