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Leica Thunder

Thunder widefield microscope features the Leica technology Computational Clearing. It efficiently removes out-of-focus blur in real time, enabling the meaningful use of 3D specimens with camera-based fluorescence microscopes. The high sensitivity of the system ensures low phototoxicity and photobleaching, i.e., higher throughput with optimal conditions.

Fluorophore selection guide

5x N Plan
• Numerical Aperture: 0.12
• Working Distance: 14.0 mm
• Immersion: Without immersion

10x HC Plan Apo
• Numerical Aperture: 0.45
• Working Distance: 2.8 mm
• Immersion: Without immersion

20x IMM UV
• Numerical Aperture: 0.7
• Working Distance: 0.26 mm
• Immersion: Water Glyc Oil

20x Plan Apo
• Numerical Aperture: 0.80
• Working Distance: 0.4 mm
• Immersion: Without immersion

16x HC Fluotar L VISIR
• Numerical Aperture: 0.60
• Working Distance: 2.5 mm
• Immersion: IMM incl BABB Water, Sil-oil Glyc

100x HCX Plan Apo
• Numerical Aperture: 1.4
• Working Distance: 0.09 mm
• Immersion: Oil

Leica LED8.
  • -8 solid state sources operating independently
  • -395, 438, 475, 511, 555, 575, 635, 730 nm
    • External filter wheel.
  • -Filter exchange time 21ms
  • -Filter exchange time by microscope turret is 300ms
  • The Thunder is equipped with 2 cameras.
    • Leica K8 for fluorescence imaging and Leica K3C for colour imaging.

  • Leica K8 camera
  • -18.8 mm sensor, 2048x2048 pix, Pixel 6.5 μm x 6.5 μm
  • -QE 95% (peak)
  • -Dark current noise 1.5 e-/pix/sec
  • -Read noise: 12-bit 1.0 e- (median) 1.1 e- (RMS)

    • Leica K3C camera
  • -color CMOS camera
  • -excellent color reproduction
  • -12-bit/channel RGB quantifiable images
    • LAS X with Navigator.
  • -Raw data is saved always separately
  • -Image processing can be done as a part of an experiment or by post-processing