CRISPR workshop 2024

    Turku Bioscience Genome Editing Core is organizing a workshop on generating knock-out and knock-in modification using CRISPR/Cas9 technology with in vitro ribonucleoprotein complex delivery. During the course, participants will learn how to create specific genetic modifications with the CRISPR/Cas9 system, aspects of designing gRNAs and homology-directed repair templates, and they will gain hands-on experience by working with 293T cell lines. Participants will use their own laptop to design specific guideRNAs targeting their favorite gene of interest.


    Introduction to CRISPR/Cas9 system
    gRNA and homology-directed repair template design
    in vitro ribonucleoprotein delivery
    Experimental efficiency analysis

    Program and timetable:

    18.3. Theoretical background, CRISPR experiment design
    19.3. RNP assembly and nucleofection
    22.3. Cell harvest and flow cytometry, data analysis, CRISPR experiment design task processing


    Medisiina D, C209 and C235
    University of Turku,
    Kiinamyllynkatu 10,
    20520, Turku


    PhD students, post-doctoral researchers, researchers
    Maximum number of participants: 20


    For UTU members: 100 €
    Others: 100 € + VAT (24%)

    Registration deadline:

    Registration via Konsta.

    Further information: