Instruments and technologies
Chromium X
(10x Genomics)
- Compatible with all current single cell assays, low- to high-throughput
- 1-16 samples and >700 000 single cells in one run
Chromium Connect
(10x Genomics)
- One-day, fully automated single-cell capture and RNA-seq library preparation
- Extremely high sample-to-sample consistency
- Compatible with 3’- and 5’-scRNA-seq protocols
Chromium Controller
(10x Genomics)
- Standard kit:
1-8 samples per run, 500 – 10 000 cells per sample - Low-throughput kit:
1-4 samples per run, <1 000 cells per sample
Visium
(10x Genomics)
- Spatial transcriptomics and proteomics from tissue sections
- Compatible with fresh frozen and FFPE sections
- Next generation sequencing as a readout
https://www.10xgenomics.com/products/spatial-gene-expression
GeoMx Digital Spatial Profiler
(Nanostring)
- Spatial transcriptomics and proteomics from tissue sections
- Hybridisation-based analysis
- Regions of Interest (ROIs) can be flexibly defined (10-700 μm)
- Next generation sequencing as a readout
https://www.nanostring.com/products/geomx-digital-spatial-profiler/geomx-dsp-overview/
Luna FX7 Automated Cell Counter
(Logos Biosystems)
- Fluorescence cell counter
- We strongly encourage performing final cell counts using Luna FX7 for single cell experiments
https://logosbio.com/automated-cell-counters/fluorescence/luna-fx7
Helios CyTOF (Fluidigm)
Our instrument is Helios, a 3rd generation CyTOF system, which has 135 detection channels.
The Cell Imaging and Cytometry (CIC) Core gives support in panel design, panel assembly, stainings and data analysis. Data acquisition is performed by CIC personnel.
A recent review of the technology: Stern et al., 2018.
For further information, please contact masscytometry@bioscience.fi