Instruments and technologies

    Chromium X
    (10x Genomics)

    Chromium X
    • Compatible with all current single cell assays, low- to high-throughput
    • 1-16 samples and >700 000 single cells in one run

    https://www.10xgenomics.com/instruments/chromium-x-series

    Chromium Connect
    (10x Genomics)

    Chromium Connect
    • One-day, fully automated single-cell capture and RNA-seq library preparation
    • Extremely high sample-to-sample consistency
    • Compatible with 3’- and 5’-scRNA-seq protocols

    https://www.10xgenomics.com/instruments/chromium-connect

    Chromium Controller
    (10x Genomics)

    Chromium Controller
    • Standard kit:
      1-8 samples per run, 500 – 10 000 cells per sample
    • Low-throughput kit:
      1-4 samples per run, <1 000 cells per sample

    https://www.10xgenomics.com/instruments/chromium-controller


    Visium
    (10x Genomics)

    Visium
    • Spatial transcriptomics and proteomics from tissue sections
    • Compatible with fresh frozen and FFPE sections
    • Next generation sequencing as a readout

    https://www.10xgenomics.com/products/spatial-gene-expression

    GeoMx Digital Spatial Profiler
    (Nanostring)

    GeoMx Digital Spatial Profiler
    • Spatial transcriptomics and proteomics from tissue sections
    • Hybridisation-based analysis
    • Regions of Interest (ROIs) can be flexibly defined (10-700 μm)
    • Next generation sequencing as a readout

    https://www.nanostring.com/products/geomx-digital-spatial-profiler/geomx-dsp-overview/

    Luna FX7 Automated Cell Counter
    (Logos Biosystems)

    Luna FX7
    • Fluorescence cell counter
    • We strongly encourage performing final cell counts using Luna FX7 for single cell experiments

    https://logosbio.com/automated-cell-counters/fluorescence/luna-fx7

    Helios CyTOF (Fluidigm)

    Our instrument is Helios, a 3rd generation CyTOF system, which has 135 detection channels.
    The Cell Imaging and Cytometry (CIC) Core gives support in panel design, panel assembly, stainings and data analysis. Data acquisition is performed by CIC personnel.
    A recent review of the technology: Stern et al., 2018.

    For further information, please contact masscytometry@bioscience.fi