Instruments and technologies
- Chromium X (10x Genomics)
- Chromium Connect (10x Genomics)
- Chromium Controller (10x Genomics)
- Visium
- GeoMx Digital Spatial Profiler (Nanostring)
- Luna FX7 Automated Cell Counter (Logos Biosystems)
- Helios CyTOF (Fluidigm)
- Compatible with all current single cell assays, low- to high-throughput
- 1-16 samples and >700 000 single cells in one run
- https://www.10xgenomics.com/instruments/chromium-x-series
One-day, fully automated single-cell capture and RNA-seq library preparation- Extremely high sample-to-sample consistency
- Compatible with 3’- and 5’-scRNA-seq protocols
- https://www.10xgenomics.com/instruments/chromium-connect
- Standard kit: 1-8 samples per run, 500 - 10 000 cells per sample
- Low-throughput kit: 1-4 samples per run, <1 000 cells per sample
- https://www.10xgenomics.com/instruments/chromium-controller
Spatial transcriptomics and proteomics from tissue sections- Compatible with fresh frozen and FFPE sections
- Next generation sequencing as a readout
- https://www.10xgenomics.com/products/spatial-gene-expression
Spatial transcriptomics and proteomics from tissue sections- Hybridisation-based analysis
- Regions of Interest (ROIs) can be flexibly defined (10-700 μm)
- Next generation sequencing as a readout
- https://www.nanostring.com/products/geomx-digital-spatial-profiler/geomx-dsp-overview/
- Fluorescence cell counter
- We strongly encourage performing final cell counts using Luna FX7 for single cell experiments
- https://logosbio.com/automated-cell-counters/fluorescence/luna-fx7
- Our instrument is Helios, a 3rd generation CyTOF system, which has 135 detection channels.
- The Cell Imaging and Cytometry (CIC) Core gives support in panel design, panel assembly, stainings and data analysis. Data acquisition is performed by CIC personnel.
- A recent review of the technology: Stern et al., 2018.
- For further information, please contact cytometry [at] bioscience.fi