BD LSR Fortessa analyzers

BD LSRFortessas (two optically identical instruments) are equipped with four lasers (405nm, 488nm, 561nm, 640nm). It can be used to detect up to 17 fluorescent parameters. In addition to the BD™ High Throughput sampler, single tube acquisition is also possible.

Default optical configuration

Location: BioCity, 5th floor, room 5026

Excitation optics:
Air-cooled Coherent™ Sapphire™ solid state diode blue laser (488nm, 50mW)
Air-cooled JDS Uniphase™ solid state diode red laser (640nm, 40mW)
Air-cooled solid state diode violet laser (405nm, 50mW)
Air-cooled solid state diode yellow-green laset (561nm, 50mW)

Blue laser – octagon PMT array
Optical array for detection of signals after excitation at 488 nm
2 PMT’s for detection of fluorescence parameters and 1 PMT for SSC
Typical fluorochromes: FITC/GFP and PerCP/PerCP-Cy5.5

Red laser – trigon PMT array
Optical array for detection of signals after excitation at 640 nm
3 PMT’s for detection of fluorescence parameters
Typical fluorochromes: APC, Alexa Fluor™ 647, Alexa Fluor™ 700, APC-Cy7, BD APCH7

Violet laser – octagon PMT array
Optical array for detection of signals after excitation at 405 nm
7 PMT’s for detection of fluorescence parameters,
Typical fluorochromes: BD Horizon Brilliant Violet, 421, BD Horizon™ V450,
BD Horizon™ Brilliant Violet 510, BD Horizon V500, Pacific Blue, Pacific Orange, BD
Horizon™ Brilliant Violet 605, BD Horizon™ Brilliant Violet 650, BD Horizon; Brilliant
Violet 711, BD Horizon™ Brilliant Violet 786

Yellow-Green laser – octagon PMT array
Optical array for detection of signals after excitation at 561 nm
5 PMT’s for detection of fluorescence parameters,
Typical fluorochromes and fluorescent proteins: PE, RFP, mCherry, Pe-Texas-Red, PE-Cy5, PE-Cy5.5, PE-Cy7  

High Throughput Sampler
The BD™ High Throughput sampler (HTS plate loader) provides fully automated and rapid sample
aspiration. It supports standard 96-well U-, V-, and flat–bottom plates. The standard throughput mode allows for acquisition of
larger sample volumes. All sampler functions are operated through BD FACSDiVa software,
allowing the user to create customized delivery protocols with user-defined mixing, wash, and
analysis parameters. Single tube acquisition is also possible.

Software
Operating system Microsoft® Windows® 7
BD FACSDiVa™ software version 8

BD LSR II analyzer

The BD LSR II is equipped with three lasers (405, 488 and 635 nm) and can handle advanced multi-color applications. Up to eight different fluorescent probes can be measured simultaneously. In addition, the advanced flow system allows high speed sample runs. This instrument is used for many applications including FRET measurements.

The list below shows all the available bandpass filters. If you have a fluorochrome which needs a non-default filter, please contact flow(at)btk.fi.

Default optical configuration

Location: BioCity, 5th floor, room 5126

High Throughput Sampler

The BD™ High Throughput sampler (HTS plate loader) provides fully automated and rapid sample
aspiration. It supports standard 96-well U-, V-, and flat–bottom plates. The standard throughput mode allows for acquisition of
larger sample volumes. All sampler functions are operated through BD FACSDiVa software,
allowing the user to create customized delivery protocols with user-defined mixing, wash, and
analysis parameters. Single tube acquisition is also possible.

Sony SH800 Cell Sorter

The benchtop SH800S cell sorter permits sorting of a wide range of cell sizes for many applications using the 70μm, 100μm, and 130μm microfluidic sorting chips. This novel, chip-based design is fully integrated with comprehensive fluidics controls and advanced automation for set-up, acquisition, sort and analysis to make sorting less subjective, more precise and easier to perform.

The instrument has 4 laser lines. 6 colors + FSC and BSC (SSC) can be detected simultaneously. The use of co-linear lasers provides flexibility with fluorochrome selection.

The sorter is placed in a biosafety level 2 cabinet which allows e.g. sorting of primary cells.