Getting started

    1. Sample information

    Please, contact Genome Editing Core to first have a consultation session on your project. You will be given the required paperwork during these sessions.

    Required information include:

    • Contact persons for the project
    • Complete vector maps for plasmids to be used
    • Possible antibiotic resistances for bacterial amplification and in eucaryotic cells
    • Cell lines in question and what modifications they are to go through
    • Preferred analysis method to confirm the success of genetic modification (performed by customer)
    • Detailed culture protocols for cells Genome Editing Core staff are not used to
    • Description of how much virus vector stock is to be produced
    • Details of methods (e.g. cell sorting or transduction conditions) successfully used in previous papers

    2. Conducting the project

    Genome Editing Core staff will perform the following parts of your project:

    • Perform simple cloning tasks
    • Amplify plasmid DNA for viral vector production
    • Produce viral particles
    • Transduce cells within our viral vector laboratory
    • Perform a test to confirm the absence of viral particles to make sure it is safe to culture them outside BSL II facilities

    We expect the customer to:

    • Supply us with culture medium and supplements required for cell culture (of other than vector producer cells)
    • Perform the analysis (often by Western blot or qRT-PCR), though core staff will perform the lysis

    3. Customer feedback

    Your feedback is important to us as we are improving and expanding our services. Please fill in the feedback form.

    4. Submit your publication

    Keeping track of how we have enabled your imagination helps us expand our services in the right direction. Please fill in the form to have your study displayed on pages.