shRNA Protein Downregulation
Mammalian short hairpin RNA (shRNA) interference is a mechanism of translational control that targets expression at post-transcriptional level. The translation of particular mRNA transcripts is prevented by homologous short strands of RNA bound to an RNA-induced silencing complex (RISC). When RISC detects a sequence complementary to the shRNA, it either cleaves it or prevents its translation and marks it for degradation.
Viral vectors carrying shRNA sequences can be used for both permanent genenome editing and transient knockdown models. We at Genome Editing Core specialise in viral vector-mediated shRNA methods that guarantee high shRNA expression rates.
- Generation of stable shRNA-expressing cell lines.
- Transient shRNA knockdown of a gene.
- Inducible shRNA expression systems.
Please, contact Genome Editing Core for a consultation on your project and study design.