Transgene Overexpression

    We at Genome Editing Core specialise in viral vector-mediated transgene expression. Whether it is transient or stable expression you are after, whether you conduct your studies in vitro or in vivo, we have a vector for that! Viral vectors can be a cost-effective alternative to trasfection with expensive reagents and may achieve up to near 100% transgene expression, even in hard-to-transfect cell populations.

    If your protein of interest is cytotoxic or negatively affects proliferation, tight control of protein expression is crucial. Stable cell line generation with vectors that integrate into host genomic DNA is still possible by using various inducible systems, for instance tetracycline-dependent regulatory elements.

    We offer:

    • Full service genome editing of the cell line of your choice.
    • Fine-tuning the level of transgene expression of fluorescently-tagged cells by cell sorting.

    Please contact Genome Editing Core for consultations on study design, choice of vector, and how we can enable your imagination!