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    3i CSU-W1 spinning disk

    The 3i Marianas CSU-W1 spinning disk (50µm pinholes) confocal microscope is perfect for fast gently imaging of live samples or large fields of view. Fast z-stack acquisition together with tile options facilitates collection of large 3D data sets.

    Fluorophore selection guide

    Specialized Techniques:
    • Sensitive camera for low light live cell imaging
    • Fluorescence recovery after photobleaching (FRAP)
    • Photoactivation
    Reservation System: 3i CSU-W1 spinning disk Location: BioCity, 5th floor, room 5149
    10x Zeiss Plan-Apochromat
    • Numerical Aperture: 0.45
    • Working Distance: 2 mm
    • Immersion: Without immersion

    20x Zeiss Plan-Apochromat
    • Numerical Aperture: 0.8
    • Working Distance: 0.55 mm
    • Immersion: Without immersion

    40x Zeiss LD C-Apochromat
    • Numerical Aperture: 1.1
    • Working Distance: 0.62 mm at cover glass 0.17 mm
    • Immersion: Water

    63x Zeiss LD Plan-NEOFLUAR
    • Numerical Aperture: 0.75
    • Working Distance: 1.7 mm at cover glass 0.75 mm
    • Immersion: Without immersion
    63x Zeiss LD C-Apochromat
    • Numerical Aperture: 1.15
    • Working Distance: 0.6 mm at cover glass 0.17 mm
    • Immersion: Water

    63x Zeiss Plan-Apochromat
    • Numerical Aperture: 1.4
    • Working Distance: 0.19 mm
    • Immersion: Oil

    100x Zeiss Plan-Apochromat
    • Numerical Aperture: 1.4
    • Working Distance: 0.17 mm
    • Immersion: Oil
    Solid state lasers:
    • 405nm - 100mW
    • 488nm - 150mW
    • 561nm - 100mW
    • 640nm - 100mW
    • 730nm - 40mW
    • Dapi   (445/45nm)
    • GFP (525/50) - previously 525/30nm before 18.08.21
    • Cy3/Alexa 568 (617/73nm or 600/37nm)
    • Cy5/Alexa647 (692/40nm)
    • Alexa 750 IR (809/81nm)
    • Quad-band (440/40nm, 521/21nm, 607/34nm, 700/45)
    Hamamatsu sCMOS Orca Flash4.0
    2048 x 2048 pixels, 6.5x6.5 um
    • Piezo stage - ASI - 300μm working distance
    • Definite focus
    • Environment chamber - Okolab
    • Temperature control - Okolab