Total Internal Reflection (TIRF) microscopy is a technique, which allows imaging of fluorophores near of the basal cell membrane with high resolution in Z-axis. Approximately 70-150 nm -axis resolution can be achieved, while in common confocal microscopy the limit is approximately 600nm.
TIRF is designed for cell membrane studies, such as endosytosis and integrin trafficking. However,  it is very suitable for all kinds of sensitive wide field microscopy, due to high quality objectives and sensitive cameras.

Our TIRF has two different cameras dedicated to different applications: a very sensitive emccd camera with a 512×512 chip, and a sensitive and fast high resolution sCMOS camera. The microscope is equipped with incubation elements, motorized stage and Zeiss Definite Focus drift correction device.

Reservation System: Zeiss TIRF

Location: BioCity, 2nd floor, room 2209