BD LSR Fortessa analyzers
BD LSRFortessas (two optically identical instruments) are equipped with four lasers (405nm, 488nm, 561nm, 640nm). It can be used to detect up to 17 fluorescent parameters. In addition to the BD™ High Throughput sampler, single tube acquisition is also possible. Fortessa default configuration Reservation System: BD LSRFortessa Jazz BD LSRFortessa Blues Location: BioCity 5th floor, room 5160/5107
- Air-cooled solid state diode violet laser (405nm, 50mW)
- Air-cooled Coherent™ Sapphire™ solid state diode blue laser (488nm, 50mW)
- Air-cooled solid state diode yellow-green laser (561nm, 50mW)
- Air-cooled JDS Uniphase™ solid state diode red laser (640nm, 40mW)
Violet laser – octagon PMT array
Optical array for detection of signals after excitation at 405 nm
Optical array for detection of signals after excitation at 488 nm
Optical array for detection of signals after excitation at 405 nm
- 7 PMT’s for detection of fluorescence parameters
- Typical fluorochromes: BD Horizon Brilliant Violet, 421, BD Horizon™ V450, BD Horizon™ Brilliant Violet 510, BD Horizon V500, Pacific Blue, Pacific Orange, BD Horizon™ Brilliant Violet 605, BD Horizon™ Brilliant Violet 650, BD Horizon; Brilliant Violet 711, BD Horizon™ Brilliant Violet 786
Optical array for detection of signals after excitation at 488 nm
- 2 PMT’s for detection of fluorescence parameters and 1 PMT for SSC
- Typical fluorochromes: FITC/GFP and PerCP/PerCP-Cy5.5
Yellow-Green laser – octagon PMT array
Optical array for detection of signals after excitation at 561 nm
Red laser – trigon PMT array
Optical array for detection of signals after excitation at 640 nm
Optical array for detection of signals after excitation at 561 nm
- 5 PMT’s for detection of fluorescence parameters
- Typical fluorochromes and fluorescent proteins: PE, RFP, mCherry, Pe-Texas-Red, PE-Cy5, PE-Cy5.5, PE-Cy7
Red laser – trigon PMT array
Optical array for detection of signals after excitation at 640 nm
- 3 PMT’s for detection of fluorescence parameters
- Typical fluorochromes: APC, Alexa Fluor™ 647, Alexa Fluor™ 700, APC-Cy7, BD APCH7
The BD™ High Throughput sampler (HTS plate loader) provides fully automated and rapid sample
aspiration. It supports standard 96-well U-, V-, and flat–bottom plates. The standard throughput mode allows for acquisition of larger sample volumes. All sampler functions are operated through BD FACSDiVa software,
allowing the user to create customized delivery protocols with user-defined mixing, wash, and
analysis parameters.
Single tube acquisition is also possible.
Single tube acquisition is also possible.
- Operating system Microsoft® Windows® 7
- BD FACSDiVa™ software version 8