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    3i CSU-W1 Spinning disk – Med C

    The 3i Marianas CSU-W1 spinning disk (50µm pinholes) confocal microscope is perfect for fast gently imaging of live samples or large fields of view. Fast z-stack acquisition together with tile options facilitates collection of large 3D data sets.

    Specialized Techniques:
    • Sensitive camera for low light live cell imaging
    • Mesa illumination enhancement and field flattening
    Reservation System: 3i CSU-W1 Spinning disk – Med C

    Location: Medisiina C, 2nd floor, room C242
    5x Zeiss EC Plan-Neofluar
    • Numerical Aperture: 0.16
    • Working Distance: 18.5 mm
    • Immersion: Without immersion

    10x Zeiss Plan-Apochromat
    • Numerical Aperture: 0.45
    • Working Distance: 2 mm
    • Immersion: Without immersion

    20x Zeiss LD Plan-Neofluar
    • Numerical Aperture: 0.4
    • Working Distance: 7.9 mm at cover glass 0.75 mm
    • Immersion: Without immersion
    20x Zeiss Plan-Apochromat
    • Numerical Aperture: 0.8
    • Working Distance: 0.55 mm
    • Immersion: Without immersion

    40x Zeiss LD Plan-Neofluar
    • Numerical Aperture: 0.6
    • Working Distance: 2.9 mm at 0.75 glass
    • Immersion: Without Immersion

    63x Zeiss Plan-Apochromat
    • Numerical Aperture: 1.4
    • Working Distance: 0.17 mm
    • Immersion: Oil
    Solid state lasers:
    • 405nm - 100mW
    • 488nm - 150mW
    • 561nm - 150mW
    • 640nm - 100mW
    • Dapi (445/45nm)
    • GFP (525/30nm)
    • Cy3/Alexa 568 (617/73nm)
    • Cy5/Alexa647 (692/40nm)
    • Quad band (440/40nm, 521/21nm, 607/34nm, 700/45)
    Photometrics Prime BSI sCMOS
    2048 x 2048 pixels
    • Piezo stage - ASI - 300μm working distance
    • Definite focus
    • Environment chamber - Okolab
    • Temperature control - Okolab