The Nikon Eclipse Ti2-E offers improved system speed, increased flexibility and efficient multi-mode microscopy as part of a fully-integrated microscope system that is ideal for high-end research and live-cell imaging. In addition to glass-bottom dishes, plastic dishes can be used. The system is especially suited to high-content applications that involve multi-well plates and slide scanning with the choice between colour and monochromatic cameras.

    Fluorophore selection guide

    Reservation System: Nikon Eclipse Ti2-E

    Location: BioCity, 5th floor, room 5149

    2x Nikon Plan-Apochromat
    • Numerical Aperture: 0.06
    • Working Distance: 7.5 mm
    • Immersion: Without immersion

    4x Nikon Plan-Fluor
    • Numerical Aperture: 0.13
    • Working Distance: 17.2 mm
    • Immersion: Without immersion

    4x Nikon Plan Apo LambdaD
    • Numerical Aperture: 0.2
    • Working Distance: 20.0 mm
    • Immersion: Without immersion

    10x Nikon CFI Plan-Fluor
    • Numerical Aperture: 0.3
    • Working Distance: 15.2 mm
    • Immersion: Without immersion


    20x Nikon CFI S Plan Fluor ELWD
    • Numerical Aperture: 0.45
    • Working Distance: 8.2-6.9 mm
    • Immersion: Without immersion

    20x Nikon CFI Plan Apo Lambda
    • Numerical Aperture: 0.75
    • Working Distance: 1 mm
    • Immersion: Without immersion

    40x Nikon CFI S Plan Fluor ELWD ADM
    • Numerical Aperture: 0.6
    • Working Distance: 3.6-2.8 mm
    • Immersion: Without immersion

    60x Nikon CFI Plan Apo Lambda
    • Numerical Aperture: 1.4
    • Working Distance: 0.13 mm
    • Immersion: Oil

    100x Nikon Apo TIRF
    • Numerical Aperture: 1.49
    • Working Distance: 0.12 mm
    • Immersion: Oil

    The Nikon Ti2-E uses Lumencor Spectra X LED excitation.
    • 395/25nm
    • 440/20nm
    • 475/28nm
    • 550/15nm
    • 575/35nm - by request
    • 640/30nm
    • 730/40nm
    Transmitted light
    • Adjustable polariser
    Lower Deck - LED excitation
    • 89403bs – CFP/YFP/mCherry/Cy7
    • 84000v2 – DAPI/ FITC/TRITC/Cy5
    • 96377 – Cy7-B
    • DIC - Fixed Analyser
    Hamamatsu ORCA sCMOS emission filters
    • DAPI sPx 435/26nm
    • CFP 475/20nm
    • GFP sPx 515/30nm
    • YFP 540/21nm
    • TRITC 595/40nm
    • mCherry 632/60nm
    • Cy5 sPx 705/71nm
    • Cy7 sPx 809/81nm - by request
    Hamamatsu sCMOS Orca Flash4.0
    2048 x 2044 pixels
    NikonDS-Fi3 CMOS (colour)
    2880 x 2048 pixels