The ONI Nanoimager is powerful superresolution microscope capable for
    • 2D and 3D single-molecule localization microscopy (SMLM)
    • 3D single-particle tracking (SPT)
    • Total internal reflection fluorescence (TIRF)
    • Förster resonance energy transfer (FRET)
    • ONI homepage
    • Olympus UPLXAPO100XO 100x/1.45
    • Oil immersion
    • WD 0.13mm
    • Violet: 405nm (150mW)
    • Blue: 488nm (1000mW)
    • Green: 561nm (500mW)
    • Red: 640nm (100mW)
    • channel 0: 498­-551 nm (band 1), 576­-620 nm (band 2)
    • channel 1: 665-705 nm
    • Hamamatsu ORCA-Flash4.0 V3
    • Digital sCMOS camera
    • 100fps full frame
    • NimOS microscope control software
    • CODI analysis software