Publications

Utilizing Macrophages Missile for Sulfate-Based Nanomedicine Delivery in Lung Cancer Therapy

Chang Liu et al.

Research (Wash D C). 2024 Aug 6;7:0448. doi: 10.34133/research.0448. eCollection 2024.

Nanomaterial-based drug delivery systems are susceptible to premature drug leakage and systemic toxicity due to lack of specific targeting, and live-cell drug delivery is also prone to be restricted by drug carrier-cell interactions. Here, a method is established to adsorb drug-loaded nanomaterials externally to the live cells, which reduces cytotoxicity caused by drug uptake and improves the bioactivity of the carrier cells and drug release at the lesion site. It was found that polyphenols act...

Gene Regulatory Network Analysis of Decidual Stromal Cells and Natural Killer Cells

Kalle T Rytkönen et al.

Reprod Sci. 2024 Oct;31(10):3159-3174. doi: 10.1007/s43032-024-01653-1. Epub 2024 Aug 1.

Human reproductive success relies on the proper differentiation of the uterine endometrium to facilitate implantation, formation of the placenta, and pregnancy. This process involves two critical types of decidual uterine cells: endometrial/decidual stromal cells (dS) and uterine/decidual natural killer (dNK) cells. To better understand the transcription factors governing the in vivo functions of these cells, we analyzed single-cell transcriptomics data from first-trimester terminations of...

Synaptic proteome perturbations after maternal immune activation: Identification of embryonic and adult hippocampal changes

Anna Y Yotova et al.

Brain Behav Immun. 2024 Oct;121:351-364. doi: 10.1016/j.bbi.2024.07.040. Epub 2024 Jul 31.

CONCLUSIONS: Our data emphasise the dynamic effects of viral-like MIA on developing and mature hippocampi and provide novel targets for study following prenatal immune challenges. The 22 proteins that changed directionality from the embryonic to adult hippocampus, suggestive of compensatory over-adaptions, are particularly attractive for future investigations.

Inferring Tree-Shaped Single-Cell Trajectories with Totem

António G G Sousa et al.

Methods Mol Biol. 2024;2812:169-191. doi: 10.1007/978-1-0716-3886-6_9.

Single-cell transcriptomics allows unbiased characterization of cell heterogeneity in a sample by profiling gene expression at single-cell level. These profiles capture snapshots of transient or steady states in dynamic processes, such as cell cycle, activation, or differentiation, which can be computationally ordered into a "flip-book" of cell development using trajectory inference methods. However, prediction of more complex topology structures, such as multifurcations or trees, remains...

HSV-1 and Cellular miRNAs in CSF-Derived Exosomes as Diagnostically Relevant Biomarkers for Neuroinflammation

Christian Scheiber et al.

Cells. 2024 Jul 17;13(14):1208. doi: 10.3390/cells13141208.

Virus-associated chronic inflammation may contribute to autoimmunity in a number of diseases. In the brain, autoimmune encephalitis appears related to fluctuating reactivation states of neurotropic viruses. In addition, viral miRNAs and proteins can be transmitted via exosomes, which constitute novel but highly relevant mediators of cellular communication. The current study questioned the role of HSV-1-encoded and host-derived miRNAs in cerebrospinal fluid (CSF)-derived exosomes, enriched from...

The PP2A regulator IER5L supports prostate cancer progression

Jana R Crespo et al.

Cell Death Dis. 2024 Jul 18;15(7):514. doi: 10.1038/s41419-024-06907-z.

Prostate cancer exhibits high prevalence and accounts for a high number of cancer-related deaths. The discovery and characterization of molecular determinants of aggressive prostate cancer represents an active area of research. The Immediate Early Response (IER) family of genes, which regulate Protein Phosphatase 2A (PP2A) activity, has emerged among the factors that influence cancer biology. Here, we show that the less studied member of this family, Immediate Early Response 5 like (IER5L), is...