Turku Bioscience Genome Editing Core is organizing a workshop on generating knock-out and knock-in modification using CRISPR/Cas9 technology with in vitro ribonucleoprotein complex delivery. During the course, participants will learn how to create specific genetic modifications with the CRISPR/Cas9 system, aspects of designing gRNAs and homology-directed repair templates, and they will gain hands-on experience by working with 293T cell lines. Participants will use their own laptop to design specific guideRNAs targeting their favorite gene of interest.

Content:

Introduction to CRISPR/Cas9 system
gRNA and homology-directed repair template design
in vitro ribonucleoprotein delivery
Experimental efficiency analysis

Program and timetable:

18.3. Theoretical background, CRISPR experiment design
19.3. RNP assembly and nucleofection
22.3. Cell harvest and flow cytometry, data analysis, CRISPR experiment design task processing

Place:

Medisiina D, C209 and C235
University of Turku,
Kiinamyllynkatu 10,
20520, Turku

Participants:

PhD students, post-doctoral researchers, researchers
Maximum number of participants: 20

Price:

For UTU members: 100 €
Others: 100 € + VAT (24%)

Registration deadline:

10.3.2024.
Registration via Konsta.

Further information:

gene_editing@biocience.fi