Event Details

Turku Bioscience Genome Editing Core is organizing a workshop on generating knock-out and knock-in modification using CRISPR/Cas9 technology with in vitro ribonucleoprotein complex delivery. During the course, participants will learn how to create specific genetic modifications with the CRISPR/Cas9 system, aspects of designing gRNAs and homology-directed repair templates, and they will gain hands-on experience by working with 293T cell lines. Participants will use their own laptop to design specific guideRNAs targeting their favorite gene of interest.

Content:

• Introduction to CRISPR/Cas9 system
• gRNA and homology-directed repair template design
• in vitro ribonucleoprotein delivery
• Experimental efficiency analysis

Program and timetable:
23.3. Theoretical background, CRISPR experiment design
24.3. RNP assembly and nucleofection
25.3. Consultation
26.3. CRISPR experiment design task processing
27.3. Cell harvest and flow cytometry, data analysis

Place:
Medisiina C, C209 and C235
University of Turku,
Kiinamyllynkatu 10,
20520, Turku

Participants:
PhD students, post-doctoral researchers, researchers

Maximum number of participants: 20

Price:

• University-affiliated: 100 € + VAT
• Industry-affiliated: 200 € + VAT

Registration:
https://eve.utu.fi/e/CRISPR_2026 open until 8 March 2026.

Further information:
gene_editing@biocience.fi