The instruments of the screening unit are listed on this page. Almost all are available for use off-line or as part of reconfigurable automation platforms. After training, most can be booked via Open Iris. Contact us for further details. Library resources are also hosted by the screening unit.
Screening instruments
- High-Throughput Microscopes/High-Content Analysers
- Integrated assay platform (PAA workcell)
- Acoustic Non-Contact Dispenser (EDC Biosystems)
- Pipetting Robot (Biomek NXP)
- Bulk Reagent Dispensers (Thermo Fisher Multidrop)
- Plate Peeler (Brooks)
- Plate Sealer-Purger (Agilent)
- Centrifuge v-spin (Agilent)
- Plate readers
- Plate washers
- Microscopes
Three of the devices are customised versions of the BD 855 Pathway High-Content Analysers, with ultra-high-power, electronically shuttered LED sources. One of these is also integrated to an automation platform with live-cell incubator and other device options, the remaining instruments are stand-alone. The fourth device recently arrived and is currently being installed.
The fifth device is a Perkin Elmer Operetta Confocal HCS Instrument equipped with Xenon arc source. Full details of all devices are available here.
This is an integrated assay platform incorporating several of the devices described below. Plates are based from the library hotel (40 plate capacity) to an acoustic dispenser for 1-to-1 or cherry picking dispense to assay plates from the 40-plate assay library hotel. The assay plates are filled with assay reagent and substrate from bulk dispensers. After a defined incubation time, the plates can be read in the integrated plate reader or the entire assay plate stack can be transferred for measurement at another device. Library plates are automatically unsealed and resealed under inert purge gas as needed.
Location: Biocity 5th floor
Contact person: Michael Courtney
The Biomek NXP can now be booked by registered users, and training requested, via Open Iris.
Location: Biocity 5th floor
Contact person: Michael Courtney
These two devices are used in automated pipelines to add reagents for specific assay steps. Available for stand-alone use or in an 80-plate integrated assay platform.
Location: Biocity 5th floor
Contact person: Michael Courtney
Location: Biocity 5th floor
Contact person: Michael Courtney
Location: Biocity 5th floor
Contact person: Michael Courtney
Location: Biocity 5th floor
Contact person: Michael Courtney
Plate Reader (BMG Clariostar Plus)
Currently, spectral fluorescence and luminescence measurement modes are available, using filter-based monochromators. Available for stand-alone use. Integrated to the 80-plate integrated assay platform..
Plate Reader (BMG Optima Polarstar)
Plate Reader (Perkin Elmer Envision)
Location: Biocity 5th floor
Contact person: Michael Courtney
Plate Washer (Thermo)
Tecan 384 Well Plate Washer
Location: Biocity 5th floor
Contact person: Michael Courtney
Scanning Confocal Microscope (Biorad Radiance)
Functioning laser scanning confocal with GAsP and other detectors and bleaching functions
Multi-Point Wide-Field Fast Imaging Microscope (Custom)
This instrument is used for facility QC and for integration to automated pipelines.
Location: Biocity
Contact person: Michael Courtney