Per- and polyfluoroalkyl substances (PFAS) are highly persistent environmental pollutants that bioaccumulate in living organisms and are increasingly implicated in metabolic dysfunction-associated steatotic liver disease (MASLD). Mitochondria, as central regulators…
Early-life proteomic and microbiome features signal obesity risk across 26 years of follow-up
Childhood obesity is rising globally. Yet, few studies have examined the microbiome and proteome in early childhood in relation to this outcome, and most are cross-sectional by design. Early-life factors…
Clarifying the scope and capabilities of ROTS in differential expression analysis
SUMMARY: Recently, Anwar et al. introduced a method combining the ROTS reproducibility optimisation procedure with empirical Bayes variance estimation from limma. Here, we clarify several methodological aspects to support accurate…
Wake-active brainstem GABA neurons signal sleep pressure by upregulating AMPA receptors to drive recovery sleep
How the brain compensates for sleep deprivation (SD) by generating recovery sleep (RS) is not understood. Using Ca²⁺ photometry, we identified a WAKE/rapid eye movement sleep (REMS)-active somatostatin/parvalbumin GABAergic population…
Joint modeling of longitudinal and time-to-event data for dynamic disease risk prediction using proteomics
Biomedical studies increasingly incorporate longitudinal data, enabling us to track individual disease processes over time at the molecular level, and to discover associations of the molecular profiles with the outcome…
Association between infant and toddler gut microbiota composition and later executive functioning
CONCLUSIONS: Alpha diversity in infancy was negatively associated with preschool EF. Additionally, EFs differed between microbial groups based on dominant genera. Bacterial genera abundances were related to some EFs, but…
Novel CRISPR-Cas9 BAP1 knockout pre-clinical tumor model recapitulates human melanoma tumorigenesis and immune evolution
BAP1-deficient melanocytic tumors exhibit strong immunosuppressive features and poor prognosis. Currently, no immune-competent preclinical models exist to study their tumor-immune interactions or test new immunotherapies. This limitation hinders progress in…
Gene-programmed micro-nano metabolic engine drives coupled osteogenic-angiogenic regeneration
Effective bone healing is accompanied by coupled osteogenic-angiogenic regeneration, a process that critically depends on metabolic communication between bone marrow mesenchymal stem cells (BMSCs) and endothelial cells through an endogenous…
Feasibility of multimodal metabolic analysis for detecting early changes in acute neuroinflammation
Given the prevalence of metabolic perturbations in a variety of neurological and neurodegenerative diseases, understanding and monitoring brain metabolism is a key step in our advancement of therapies. The details…
Targeting Sphingosine-1-Phosphate Signaling Attenuates Doxorubicin-Aggravated Bone Loss in Obese Breast Cancer Mice
Although chemotherapy-induced bone loss is well-recognized during breast cancer treatment, the underlying mechanism remains to be further elucidated, especially in patients with obesity. In this study, the objective was to…