How the brain compensates for sleep deprivation (SD) by generating recovery sleep (RS) is not understood. Using Ca²⁺ photometry, we identified a WAKE/rapid eye movement sleep (REMS)-active somatostatin/parvalbumin GABAergic population…
Joint modeling of longitudinal and time-to-event data for dynamic disease risk prediction using proteomics
Biomedical studies increasingly incorporate longitudinal data, enabling us to track individual disease processes over time at the molecular level, and to discover associations of the molecular profiles with the outcome…
Association between infant and toddler gut microbiota composition and later executive functioning
CONCLUSIONS: Alpha diversity in infancy was negatively associated with preschool EF. Additionally, EFs differed between microbial groups based on dominant genera. Bacterial genera abundances were related to some EFs, but…
Novel CRISPR-Cas9 BAP1 knockout pre-clinical tumor model recapitulates human melanoma tumorigenesis and immune evolution
BAP1-deficient melanocytic tumors exhibit strong immunosuppressive features and poor prognosis. Currently, no immune-competent preclinical models exist to study their tumor-immune interactions or test new immunotherapies. This limitation hinders progress in…
Gene-programmed micro-nano metabolic engine drives coupled osteogenic-angiogenic regeneration
Effective bone healing is accompanied by coupled osteogenic-angiogenic regeneration, a process that critically depends on metabolic communication between bone marrow mesenchymal stem cells (BMSCs) and endothelial cells through an endogenous…
Feasibility of multimodal metabolic analysis for detecting early changes in acute neuroinflammation
Given the prevalence of metabolic perturbations in a variety of neurological and neurodegenerative diseases, understanding and monitoring brain metabolism is a key step in our advancement of therapies. The details…
Targeting Sphingosine-1-Phosphate Signaling Attenuates Doxorubicin-Aggravated Bone Loss in Obese Breast Cancer Mice
Although chemotherapy-induced bone loss is well-recognized during breast cancer treatment, the underlying mechanism remains to be further elucidated, especially in patients with obesity. In this study, the objective was to…
REACTOR: REgulon Activity analysis and Comparison Tool for single-cell transcriptOmics Research
SUMMARY: We introduce REACTOR, a computational tool designed to detect differential activity of transcriptional regulators and their target genes (regulons) in single-cell RNA-sequencing data. It expands the currently available framework…
Fibrillar adhesion dynamics govern the timescales of nuclear mechano-response via the vimentin cytoskeleton
The cell nucleus is continuously exposed to external signals, of both chemical and mechanical nature. To ensure proper cellular response, cells need to regulate the transmission, timing and duration of…