Tumor border configuration influences colorectal cancer (CRC) prognosis, yet its molecular determinants remain unclear and existing assessment criteria have faced challenges with reproducibility. We introduce the Tumor Invasive Border Index…
Inactive β1-integrin acts as a junctional scaffold for angiopoietin/TIE2/FOXO1 signaling
The blood and lymphatic vascular systems are regulated by angiopoietin (ANGPT) growth factors, which signal via endothelial TIE receptor tyrosine kinases and integrins. However, mechanistic understanding of how these receptors…
Prognostic role of histological depth of invasion in T1-2 oropharyngeal squamous cell carcinoma
CONCLUSION: DOI was not a prognostic factor in p16-positive T1-2 OPSCC. Nevertheless, DOI may hold prognostic relevance for p16-negative disease. The patterns of local invasion and locoregional spread in OPSCC…
PFAS exposure modulates mitochondrial susceptibility in steatotic hepatocytes
Per- and polyfluoroalkyl substances (PFAS) are highly persistent environmental pollutants that bioaccumulate in living organisms and are increasingly implicated in metabolic dysfunction-associated steatotic liver disease (MASLD). Mitochondria, as central regulators…
Early-life proteomic and microbiome features signal obesity risk across 26 years of follow-up
Childhood obesity is rising globally. Yet, few studies have examined the microbiome and proteome in early childhood in relation to this outcome, and most are cross-sectional by design. Early-life factors…
Clarifying the scope and capabilities of ROTS in differential expression analysis
SUMMARY: Recently, Anwar et al. introduced a method combining the ROTS reproducibility optimisation procedure with empirical Bayes variance estimation from limma. Here, we clarify several methodological aspects to support accurate…
Wake-active brainstem GABA neurons signal sleep pressure by upregulating AMPA receptors to drive recovery sleep
How the brain compensates for sleep deprivation (SD) by generating recovery sleep (RS) is not understood. Using Ca²⁺ photometry, we identified a WAKE/rapid eye movement sleep (REMS)-active somatostatin/parvalbumin GABAergic population…
Joint modeling of longitudinal and time-to-event data for dynamic disease risk prediction using proteomics
Biomedical studies increasingly incorporate longitudinal data, enabling us to track individual disease processes over time at the molecular level, and to discover associations of the molecular profiles with the outcome…
Association between infant and toddler gut microbiota composition and later executive functioning
CONCLUSIONS: Alpha diversity in infancy was negatively associated with preschool EF. Additionally, EFs differed between microbial groups based on dominant genera. Bacterial genera abundances were related to some EFs, but…
Novel CRISPR-Cas9 BAP1 knockout pre-clinical tumor model recapitulates human melanoma tumorigenesis and immune evolution
BAP1-deficient melanocytic tumors exhibit strong immunosuppressive features and poor prognosis. Currently, no immune-competent preclinical models exist to study their tumor-immune interactions or test new immunotherapies. This limitation hinders progress in…