CONCLUSIONS: We demonstrate that miRNA gene transcriptional activity is widely impacted in immune cells by aging and during disease development and further identify the corresponding translatome signature of inflamed adipose…
Functional characterization and directed evolution of Cicer arietinum glutathione transferases for enhanced hydroperoxidase activity and ligandin function
Tau class glutathione transferases (GSTUs) play essential roles in plant defense by facilitating the nucleophilic attack of glutathione (GSH) to a wide range of electrophilic xenobiotics. In addition to their…
Early life gut microbial and metabolic shifts reflected in stool consistency- a gut transit time proxy
Transit time, fluid intake, diet, and overall gut health all influence stool consistency. However, the relationships between the gut microbiota, metabolome (microbial metabolites), and stool consistency in infants and young…
Anisomelic acid promotes proteasomal degradation of HPV16 E6 via E3 ligase recruitment: A mass spectrometry-based interactome study
Human papillomavirus (HPV) is a major driver of cervical and other epithelial cancers, with the viral oncoprotein E6 playing a central role in tumorigenesis by promoting degradation of the tumor…
HSF2 drives breast cancer progression by acting as a stage-specific switch between proliferation and invasion
Breast cancer is hallmarked by phenotypic transitions enabling abnormal cell proliferation and invasion. The stress-protective transcription factor heat shock factor 2 (HSF2) is associated with cancer, but its function in…
Breast Milk Proteome: Changes in the Different Stages of Lactation and Impacts of Gestational Diabetes Mellitus and Body Mass Index
Breast milk proteome comprises hundreds of bioactive proteins supporting infant development. The extent to which maternal metabolic conditions modify the proteome is poorly known. This study investigates proteome evolution from…
Metabolome informs about the chemical exposome and links to brain health
The metabolome is an intermediate phenotype, summarizing the profile of all small molecules (…
Designing Fluorescent Estrogen Mimetic 7-hydroxycoumarin Probe Substrates for Human Sulfotransferase Enzymes
Sulfonation is one of drug metabolism reactions affecting homeostasis of estrogens. C-3 aryl substituted 7-hydroxycoumarins are fluorescent estrogen mimetics; i.e., the hydroxyl groups of both estrogens and 7-hydroxycoumarins are conjugated…
Cholesterol Ester Storage Disease in Two Field Spaniels With Lysosomal Acid Lipase Deficiency
Cholesterol ester storage disease (CESD) is a rare genetic lysosomal storage disorder resulting from lower lysosomal acid lipase (LAL) activity. LAL is an essential enzyme required in intracellular lipid metabolism,…
The impact of conjugation strategies and linker density on the performance of the Spermine-AcDex nanoparticle-splenocyte conjugate
A common approach in living medicine engineering is modifying cell surfaces with nanomedicines to form nanoparticle-cell conjugates. Despite various available strategies, limited research has examined how conjugation strategies affect the…