Customizable FDM-based zebrafish larvae mold for live imaging 

    Marcela Xiomara Rivera Pineda et al.

    Biol Open. 2026 Mar 13:bio.062406. doi: 10.1242/bio.062406. Online ahead of print.

    Published on March 13, 2026

    Abstract

    ABSTRACT

    Accurate and reproducible orientation of zebrafish larvae is essential for high-resolution live imaging but remains difficult with standard agarose mounting. Although previous methods have used 3D-printed tools, often fabricated via stereolithography (SLA), here we present an orientation tool made via fused deposition modeling (FDM) and enhanced with a thin resin coating to improve surface smoothness and performance. This design creates consistent, larval-shaped wells that orient larvae dorsoventrally. The molds, designed to fit standard glass-bottom imaging dishes, are inexpensive to produce on common desktop 3D printers and can be easily customized to fit different imaging setups using the open-source design files. This approach enables reliable, long-term live imaging of multiple larvae and extends the use of low-cost additive manufacturing in zebrafish imaging workflows.

    PMID:41823049 | DOI:10.1242/bio.062406

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