Detection of Tn-antigen in breast and prostate cancer models by VVL-labeled red dye-doped nanoparticles
Nanomedicine (Lond). 2024;19(29):2463-2478. doi: 10.1080/17435889.2024.2405454. Epub 2024 Oct 9.
Published on October 9, 2024
ABSTRACT
Aim: Fluorescence detection of breast and prostate cancer cells expressing Tn-antigen, a tumor marker, with Vicia villosa lectin (VVL)-labeled nanoparticles.Materials & methods: Breast and prostate cancer cells engineered to express high levels of Tn-antigen and non-engineered controls were incubated with VVL-labeled or unlabeled red dye-doped silica-coated polystyrene nanoparticles. The binding to cells was studied with flow cytometry, confocal microscopy, and electron microscopy.Results: Flow cytometry showed that the binding of VVL-labeled nanoparticles was significantly higher to Tn-antigen-expressing cancer cells than controls. Confocal microscopy demonstrated that particles bound to the cell surface. According to the correlative light and electron microscopy the particles bound mostly as aggregates.Conclusion: VVL-labeled nanoparticles could provide a new tool for the detection of Tn-antigen-expressing breast and prostate cancer cells.
PMID:39382009 | PMC:PMC11520574 | DOI:10.1080/17435889.2024.2405454