Protocol to study early lung developmental branching in mouse embryos using explant culture 

    Salli Talvi et al.

    STAR Protoc. 2024 Sep 20;5(3):103198. doi: 10.1016/j.xpro.2024.103198. Epub 2024 Jul 17.

    Published on July 18, 2024

     

    ABSTRACT

    Mouse lung branching morphogenesis creates epithelial tree structures required for respiration. Here, we present a protocol for studying mouse lung developmental branching using lung explant cultures. We describe steps for isolating lungs with a video at embryonic day 12.5 (E12.5) and culturing as an explant for 2 days. We also detail procedures for microscopic imaging on days 0-2 and analysis of peripheral lung buds. This technique has the potential to investigate lung development in various conditions. For complete details on the use and execution of this protocol, please refer to Talvi et al.1.

    PMID:39024094 | PMC:PMC11315079 | DOI:10.1016/j.xpro.2024.103198

     Read More

    Back to Publications
    Recent Publication
    Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish 
    Anni K Saralahti et al.FEBS Open Bio. 2026 Jul 31:10.1002/2211-5463.70320. doi: 10.1002/2211-5463.70320. Online ahead of print.
    Recent Publication
    Nutrient intake, gut inflammation markers, and intestinal permeability in infants aged 6-12 months 
    Tuuli E I Salo et al.Clin Nutr. 2026 Jul 22;64:106739. doi: 10.1016/j.clnu.2026.106739. Online ahead of print.
    Recent Publication
    Unbiased discovery of autoreactive type 1 diabetes T-cell receptors that bind specific hybrid insulin peptides 
    Rebecca Elyanow et al.Res Sq [Preprint]. 2026 Jul 14:rs.3.rs-10074672. doi: 10.21203/rs.3.rs-10074672/v1.
    Recent Publication
    CRISPR activation of DLX5 drives neural progenitors to the GnRH cell fate 
    Shrinidhi Madhusudan et al.J Mol Endocrinol. 2026 Jul 1;77(1):e260040. doi: 10.1530/JME-26-0040.
    Recent Publication
    Maternal Intake of Vitamins A, C, D, and E During Pregnancy and Risk of Type 1 Diabetes in the Child: The Norwegian Mother, Father, and Child Cohort Study 
    Markus Mattila et al.Am J Clin Nutr. 2026 Jul 10;124(3):101431. doi: 10.1016/j.ajcnut.2026.101431. Online ahead of print.

    More Publications

    Related Publication
    Functional Interplay between Heat Shock Protein 90 (HSP90) and Heat Shock Factors (HSFs) 
    Related Publication
    Reinstating Niche Failure in Diabetic Cranial Defects via Chronotaxic Signal-Amplifying Fluidic Biomimetic Hydrogel 
    Related Publication
    Empirically establishing drug exposure records directly from untargeted metabolomics data