Turku Bioscience Genome Editing Core is organizing a workshop on generating knock-out and knock-in modification using CRISPR/Cas9 technology with in vitro ribonucleoprotein complex delivery. During the course, participants will learn how to create specific genetic modifications with the CRISPR/Cas9 system, aspects of designing gRNAs and homology-directed repair templates, and they will gain hands-on experience by working with 293T cell lines. Participants will use their own laptop to design specific guideRNAs targeting their favorite gene of interest.

Content:

Introduction to CRISPR/Cas9 system

gRNA and homology-directed repair template design

in vitro ribonucleoprotein delivery

Experimental efficiency analysis

Program and timetable:

24.3. Theoretical background, CRISPR experiment design

25.3. RNP assembly and nucleofection

26.3. Consultation

27.3. CRISPR experiment design task processing

28.3. Cell harvest and flow cytometry, data analysis

Place:

Medisiina D, C209 and C235

University of Turku,

Kiinamyllynkatu 10,

20520, Turku

Participants:

PhD students, post-doctoral researchers, researchers

Maximum number of participants: 20

Price:

For UTU members: 100 €

Others: 100 € + VAT

Registration deadline:

19.3.2025.

https://konsta.utu.fi/Default.aspx?tabid=88&tap=21219

Further information:

gene_editing[at]bioscience.fi