Type 1 diabetes (T1D) is increasing globally, yet the earliest biological determinants remain poorly defined, particularly in general population studies. We studied the Swedish population-based ABIS birth cohort (n =…
Fast label-free live imaging with FlowVision reveals key principles of cancer cell arrest on endothelial monolayers
The rapid, transient, and unpredictable nature of interactions between circulating cells and the endothelium challenges the investigation of these events under flow conditions. Here, we developed an imaging and image-analysis…
Single-cell RNA-seq analysis of longitudinal CD4+ T cell samples reveals cell-type-specific changes during early stages of type 1 diabetes
CONCLUSIONS: Our findings reveal early, cell-type-specific changes in transcription and gene regulatory networks in CD4⁺ T cells associated with type 1 diabetes progression, highlighting key pathways and transcriptional regulators. These…
Recurrent cancer-associated ERBB4 mutations are transforming and confer resistance to targeted therapies
Receptor tyrosine kinase ERBB4 (HER4) is frequently mutated in human cancer, and ERBB4 mutations have been identified in patients relapsing on targeted therapy. Here, we addressed the functional consequences of…
PhotoFiTT: a quantitative framework for assessing phototoxicity in live-cell microscopy experiments
Phototoxicity in live-cell fluorescence microscopy can compromise experimental outcomes, yet quantitative methods to assess its impact remain limited. Here, we present PhotoFiTT (Phototoxicity Fitness Time Trial), an integrated framework combining…
A resource to empirically establish drug exposure records directly from untargeted metabolomics data
Despite extensive efforts, extracting medication exposure information from clinical records remains challenging. To complement this approach, here we show the Global Natural Product Social Molecular Networking (GNPS) Drug Library, a…
Microbiome-derived bile acid signatures in early life and their association with islet autoimmunity
Emerging studies reveal that gut microbes can conjugate diverse amino acids to bile acids, known as microbially conjugated bile acids. However, their regulation and health effects remain unclear. Here, we…
Mitochondria-Targeted Nanomotor: H2S-Driven Cascade Therapy for Hepatocellular Carcinoma
Despite advances in combination therapies for cancer treatment, most strategies rely on modular-additive designs that lack dynamic molecular cues to achieve intrinsic synergy. Herein, a mitochondrial-targeted nanoplatform is introduced that…
Regulation of cell dynamics by rapid integrin transport through the biosynthetic pathway
Constitutive integrin endocytosis and recycling control cell movement and morphology. In contrast, the role of newly synthesized integrins delivered via the biosynthetic pathway has been largely overlooked. We used the…
JNK-regulated phosphoproteome links synaptic and metabolic pathways to mood regulation
c-Jun N-terminal kinases (JNKs) are implicated in both neurodegeneration and mood regulation, including anxiety and depressive-like behaviours. Yet the consequences of JNK inhibition in vivo on protein phosphorylation in the…