Publications

Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells

James R W Conway et al.

Nat Cell Biol. 2025 May 26. doi: 10.1038/s41556-025-01663-4. Online ahead of print.

Integrins provide an essential bridge between cancer cells and the extracellular matrix, playing a central role in every stage of disease progression. Despite the recognized importance of integrin phosphorylation in several biological processes, the regulatory mechanisms and their relevance remained elusive. Here we engineer a fluorescence resonance energy transfer biosensor for integrin β1 phosphorylation, screening 96 protein tyrosine phosphatases and identifying Shp2 and PTP-PEST as negative...

Mass spectrometric insights into the protein composition of human cutaneous neurofibromas: comparison of neurofibromas with the overlying skin

Roope A Kallionpää et al.

Br J Cancer. 2025 May 20. doi: 10.1038/s41416-025-03055-9. Online ahead of print.

CONCLUSION: The cNFs are relatively quiescent, consistent with their benign nature and limited growth potential. The development of pharmacological therapy for cNFs requires overcoming the high similarity between cNFs and the overlying skin. The present dataset can serve as a resource for future research on cNFs.

High-resolution live imaging of tardigrade response to anoxia

Anna-Mari Haapanen-Saaristo et al.

Micron. 2025 May 9;196-197:103847. doi: 10.1016/j.micron.2025.103847. Online ahead of print.

Tardigrades are well-known for their ability to tolerate extreme environmental conditions such as heat, drought and lack of oxygen by undergoing cryptobiosis. The molecular responses to stress have been studied in detail, but the physiological and morphogenetic changes during cryptobiosis are less understood. We developed new live high-resolution fluorescence microscopy protocols to visualize the tardigrade response to lack of oxygen - anoxybiosis. High-resolution time-lapse imaging enabled...

CellRomeR: an R package for clustering cell migration phenotypes from microscopy data

Iivari Kleino et al.

Bioinform Adv. 2025 Apr 4;5(1):vbaf069. doi: 10.1093/bioadv/vbaf069. eCollection 2025.

MOTIVATION: The analysis of cell migration using time-lapse microscopy typically focuses on track characteristics for classification and statistical evaluation of migration behaviour. However, considerable heterogeneity can be seen in cell morphology and microscope signal intensity features within the migrating cell populations.

The ERBB2 c.1795C>T, p.Arg599Cys variant is associated with left ventricular outflow tract obstruction defects in humans

Minna Ampuja et al.

HGG Adv. 2025 May 5;6(3):100446. doi: 10.1016/j.xhgg.2025.100446. Online ahead of print.

Non-syndromic congenital heart defects (CHDs) are occasionally familial and left ventricular outflow tract obstruction (LVOTO) defects are among the subtypes with the highest hereditability. The aim of this study was to evaluate the pathogenicity of a heterozygous ERBB2 variant c.1795C>T, p.Arg599Cys identified in three families with LVOTO defects. Variant detection was done with exome sequencing. Western blotting, digital PCR, mass spectrometry (MS), MS microscopy, and flow cytometry were used...

HDAC1 is involved in the destabilization of the HSF2 protein under non-stress and stress conditions

Kevin Daupin et al.

Cell Stress Chaperones. 2025 May 1:100079. doi: 10.1016/j.cstres.2025.100079. Online ahead of print.

Heat shock transcription factors 1 and 2 (HSF1 and HSF2) are the major regulators of the cellular response to stressors, notably to heat shock and to oxidative stress. HSF1 and HSF2 are also important contributors in devasting human pathologies like cancer, neurodegenerative disorders, and neurodevelopmental disorders. Under physiological conditions, nuclear HSF2 is detected in only a few cell types in human adult healthy tissues. In contrast, HSF2 protein levels are elevated at some embryonic...